Niclosamide is a member of the class of salicylanilides. The chemical formula is C13H8Cl2N2O4. The HPLC analysis was performed using the Thermo Ultimate 3000 HPLC system. In a 60 ml round bottom flask, add 5 g of niclosamide to 15 ml ethanol and 20 ml H2O. The solubility was checked at room temperature using the HPLC analysis procedure described below. To this solution add with continuous stirring 12 g KOH pellets. Stirr until dissolved (about 10 min) then heat the reaction mixture to 50 °C while continuing to stir for 3 hours.